Research Program
Elisa Core Facility
University of Mississippi Medical Center
2500 N. State Street
Jackson, MS 39216
(601) 984-5128/5125 (phone)
(601) 984-5107 (fax)
E-mail
Elisa Core Facility in the Department of Surgery is dedicated to measure cytokines and growth factors. Cytokines and growth factors play an important regulatory role in an ever-increasing number of physiological and pathological conditions. Imbalances in cytokine and growth factor secretion due to pathogens, genetic or environmental factors result in autoimmune disorders, inflammatory diseases, allergy, cancer, metabolic and cardiovascular disorders, immunosuppression, and allograft rejection. Quantitating local or systemic production of cytokines and growth factors is extremely valuable to gain insight into the disease process and to formulate strategies to correct imbalances of these mediators. The main goal of the ECF is to provide opportunity to researchers at UMC to screen the samples at a much lower cost than commercially available Elisa kits.
Currently the following assays are available: Human: IL-1 beta, IL-6, IL-8, IL-10, IFN alpha, TGF-beta1, IFN-gamma, GM-CSF. Technical advice for sample preparation and sample storage will be available. Please contact Pratibha Joshi, Ph.D. (601-984-5128) for more information. If you are interested in assays not listed here please let us know.
Assays
Assays Human: IL-1beta, IL-6, IL-8, IL-10, IFN alpha, TGF-beta1, IFN-gamma, GM-CSF
| ASSAY | STANDARD pg/ml Sensitivity(range) |
| Human IL-1ß | 10 (15.6 - 1000) |
| Human IL-6 | 10 (7.8 - 2000) |
| Human IL-8 | 6 (3.9 - 500) |
| Human IL-10 | 10 (7.8 - 500) |
| Human TNF alpha | 2 (2 -256) |
| Human TGFbeta1 | 200 (62.5 - 4000) |
| Human IFN gamma | 25 (31.2- 4000) |
| Human GM-CSF | 10 (4.7 - 300) |
| * indicates that sensitivity is not determined yet. | |
Charges for ECF
| # of samples per assay | Cost per sample |
| 1- 50 | $4.75 |
| 51-100 | $4.50 |
| 101-150 | $4.25 |
| 151-200 | $4.00 |
| 201-250 | $3.75 |
| >250 | $3.50 |
| All samples will be run in triplicate and compared with a standard curve. | |